Imperial character story modes:
Finish the game in the Imperial storyline.
Shinsengumi character Story modes:
Finish the game in the Shinsengumi storyline.
Unlock every story mode:
Finish the game in both storylines.
Unlock the following characters in 2 player mode:
Harada Sanosuke: Finish Chapter 5
Nagakura Shinpachi: Finish Chapter 5
Hijikata Toshizo: Finish Hijikata Toshizo's story mode quest
Hirayama Gorou: Defeat Hirayama Gorou at the end of Chapter 1
Yamanami Keisuke: Defeat Yamanami Keisuke at the end of Chapter 3
Itou Kashiitarou: Defeat Itou Kashiitarou at the end of Chapter 4
Toudou Heisuke: Defeat Toudou Heisuke at the end of Chapter 4
Katsura Kogoro: Finish Katsura Kogoro's story mode quest
Kondo Isami: Finish Kondo Isami's story mode quest
Okita Souji: Finish Okita Souji's story mode quest
Saigo Takamori: Finish Saigo Takamori's story mode quest
Saito Hajime: Finish Saito Hajime repeatedly on Te-awase
Sakamato Ryoma: Finish Sakamato Ryoma's story mode quest
Serizawa Kamo: Finish Serizawa Kamo's side quest
Shinsengumi Lower Class member: Start a game on the Shinsengumi storyline
Takeda Kanryuusai: Finish the mission with Takeda Kanryuusai at Masuya
Tetsunosuke Ichimura: Meet Tetsunosuke Ichimura during Chapter 6 in Shinsengumi
Two Hitokiri: Finish the story mode for Two Hitokiri
Yamazaki Susumu: Start a game in the Shinsengumi storyline
